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Pre-treatment and Instrumental Analysis for the Separation of Bio-product
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In order to systematically reveal the pharmacological properties of the chemical components that make up natural products, it is necessary to find out not only their metabolic phenomena but also the properties of the molecular structure that make up their chemical components. The first step in determining the properties of these molecular structures must be to analyze the separation of active ingredients constituting natural products, and the interpretation of structural identification (UV, IR, NMR, mass, etc.) must be accompanied. Therefore, for the separation analysis of effective ingredients, we tried to find out the most basic sample pre-treatment method and deal with the process leading to the basics and applications of liquid chromatography. In addition, the application and interpretation of some known natural product HPLC analysis results according to the structure of chemical components were dealt with. In addition, it was intended to identify the chemical structure of various natural ingredients applied to functional foods and use them as reference materials for component analysis according to LC analysis.

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Preface? ? 1. pre-treatment of samples ?(1) the need for preprocessing ?(2) Ideal Sample Preprocessing Method ?(3) a typical preprocessing method 2. Tips for selecting reagents and solvents 3. HPLC instrumental analysis ?(1) Introduction ?(2) Basic Theory and Terms ?(3) Equipment ?(4) High Performance Liquid Chromatography(HPLC) ?(5) Preparative Liquid Chromatography 4. Analysis of amino acids ?(1) Amino Acid Structure and Role ?(2) History of Amino Acid Analysis ?(3) the field of amino acid analysis 5. Analysis of Sugar ?(1) Carbohydrates ?(2) the principle of sugar analysis 6. Ion Chromatography(IC) ?(1) Principle of Ion Chromatography ?(2) History of Ion Chromatography ?(3) Characteristics of Ion Chromatography ?(4) Applications of Ion Chromatography 7. Size Exclusion Chromatography (SEC) and Light Scattering ?(1) Size Exclusion Chromatography (SEC) ?(2) Light Scattering 8. Predictive separation of natural medicinal ingredients ?(1) Glycyrrhizae Radix ?(2) Plateycodi Radix ?(3) Angelicae Gigantis Radix ?(4) Lithospermi Radix ?(5) Cnidii Rhizoma ?(6) Astragali Radix ?(7) Phellodendri Cortex 9. Organic structure of natural products ? References? ?
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